Publications

Technical standardization of ICG near-infrared fluorescence (NIRF) laparoscopic partial nephrectomy for duplex kidney in pediatric patients

Written on 14 June 2021. Posted in Publications.

Abstract Background This study aimed to standardize the operative technique of indocyanine green (ICG) near-infrared fluorescence (NIRF) laparoscopic partial nephrectomy (LPN) and compare it with the standard technique. Methods In the last 4 years, we performed 22 LPN (14 right-sided, 8 left-sided) in children with non-functioning moiety of duplex kidney. Patients included 12 girls and 10 boys with a median age of 3.9 years (range 1–10). Patients were grouped according to the use of ICG-NIRF: G1 included 12 patients operated using ICG-NIRF and G2 included 10 patients receiving the standard...

Quantitative fluorescence angiography aids novice and experienced surgeons in performing intestinal resection in well-perfused tissue

Written on 03 May 2021. Posted in Publications.

Abstract Background Anastomotic leakage (AL) after gastrointestinal resection is a devastating complication with huge consequences for the patient. As AL is associated with poor blood supply, tools for objective assessment of perfusion are in high demand. Indocyanine green angiography (ICG-FA) and quantitative analysis of ICG-FA (q-ICG) seem promising. This study aimed to investigate whether ICG-FA and q-ICG could improve perfusion assessment performed by surgeons of different experience levels. Methods Thirteen small bowel segments with a varying degree of devascularization, including two...

Application of Indocyanine Green Fluorescence as an Adjuvant to Laparoscopic Ultrasound in Minimally Invasive Liver Resection

Written on 01 May 2021. Posted in Publications.

Abstract Background: Indocyanine green (ICG) fluorescence imaging has been extensively used in a variety of applications in visceral surgery. In minimally invasive liver resections, the detection of small superficial hepatic lesions using an intravenous injection of ICG before surgery represents a promising application. Methods: We analyzed 18 consecutive patients who underwent laparoscopic liver resection for superficial malignant tumors, namely 11 patients with hepatocellular carcinoma (HCC), 5 patients with colorectal liver metastases (CRLM), 1 patient with intrahepatic...

Nerve spectroscopy: understanding peripheral nerve autofluorescence through photodynamics

Written on 21 April 2021. Posted in Publications.

Título completo del paper: Nerve spectroscopy: understanding peripheral nerve autofluorescence through photodynamics
Autores: Fernando Dip, Rene Aleman, Mariano Socolovsky, Nerina Villalba, Jorge Falco, Emanuele Lo Menzo, Kevin P White, Raul J Rosenthal
Fecha de publicacion: 2021-12-01
doi: https://doi.org/10.1007/s00464-020-08227-7

Methods: Samples from various human peripheral nerves were selected postoperatively. Nerve fibers were divided into three groups: Group A nerve fibers were washed with a physiologic solution; Group B nerve fibers were fixated with formaldehyde for 6 h first, and then washed with a physiologic solution; Group C nerve fibers were fixated with formaldehyde for six hours, but not washed afterwards. An Olympus IX83 inverted microscope was used for close-up image evaluation. Nerve fibers were exposed to white-light wavelength spectrums for a specific time frame prior to visualization under three...

Nerve autofluorescence in near-ultraviolet light markedly enhances nerve visualization in vivo

Written on 21 April 2021. Posted in Publications.

Methods: We exposed sciatic nerves within the posterior thigh in five 250-300 gm Wistar rats, then observed them at four different NUV intensity levels: 20%, 35%, 50%, and 100%. Brightness of fluorescence was measured by fluorescence spectroscopy, quantified as a fluorescence score using Image-J software, and statistically compared between nerves, background, and both an artery and vein by unpaired Student's t tests with Bonferroni adjustment to accommodate multiple comparisons. Sensitivity, specificity, and accuracy were calculated for each NUV intensity. Results: At 20, 35, 50, and 100%...

Widespread anorectal lymphovascular networks and tissue drainage: analyses from submucosal india ink injection and indocyanine green fluorescence imaging

Written on 16 February 2021. Posted in Publications.

Methods In this descriptive study, we performed microanatomical evaluations and intraoperative imaging analysis in a cadaver and patients with rectal cancer. Specimens with India ink injection were collected from a cadaver and six patients who underwent abdominoperineal resection. Intraoperative indocyanine green fluorescence imaging was performed on four patients who underwent surgery for lower rectal cancer. India ink was injected into the submucosa at the dentate line of specimens. Tissue sections were examined by immunohistochemistry for D2‐40 and CD31. Intraoperative indocyanine...

Does Near-infrared Fluorescent Cholangiography with Indocyanine Green Reduce Bile Duct Injuries and Conversions to Open Surgery During Laparoscopic or Robotic Cholecystectomy?

Written on 16 February 2021. Posted in Publications.

Methods A thorough PubMed search was conducted to identify randomized clinical trials and nonrandomized clinical trials with ≥100 patients. Because all near-infrared fluorescent cholangiography studies were published since 2013, only studies without near-infrared fluorescent cholangiography published since 2013 were included for comparison. Incidence estimates, weighted and unweighted for study size, were adjusted for acute versus chronic cholecystitis, and for robotic versus laparoscopic cholecystectomy and are reported as events/10,000 patients. All studies were assessed for bias risk and...

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