Coordinator: Dr. Gustavo Jacob
Dr. Bruno Azevedo
Guests: Dra. Audrey Tsudona (Unicaron Medical Director)
Dr. João Paulo Epprecht

Day 1: Fundamentals and Applications of Fluorescence-Guided Surgery

7:30 - 8:30: Registration and Welcome Coffee
8:30 - 8:40: Opening and Introduction (BRUNO AZEVEDO)
• Presentation of the course objectives and an overview of the program.
8:40 - 9:30: History and Fundamentals of Fluorescence Imaging (GUSTAVO JACOB)
• Journey through the evolution of fluorescence imaging in surgical oncology, including basic concepts of fluorophores and imaging systems.
9:30 - 10:15: Understanding Fluorophores and Imaging Systems (GUSTAVO JACOB)
• Details on different fluorophores, their targets, and the operation of fluorescence imaging systems.
10:15 - 10:45: Coffee Break
10:45 - 12:45: Current Applications of Fluorescence-Guided Surgery
10:45 - 11:15: Lymphatic Imaging (BRUNO AZEVEDO)
11:15 - 11:45: Imaging Vital Structures (JOÃO PAULO EPPRECHT)
11:45 - 12:15: Tumor Tissue Imaging (GUSTAVO JACOB)
12:15 - 12:45: Tissue Perfusion Evaluation (JOÃO PAULO EPPRECHT)
12:45 - 14:00: Lunch
14:00 - 16:00: Practical Laboratory Session
14:00 - 14:45: Introduction to Bench Lab Techniques
14:45 - 15:30: Hands-on Practice with Fluorophores
15:30 - 16:00: Exploring Dilution, Depth Penetration, and Synergy between Fluorophores and Imaging Systems
16:00 - 16:30: Coffee Break
16:30 – 17:00: The Evolution of Surgery: Transitioning from 1.0 to 4.0 (AUDREY TSUNODA)
17:30 – 18:00: The International Society for Fluorescence Guided Surgery (JOÃO PAULO EPPRECHT)
• Overview of ISFGS, their mission, and how they support advancements in fluorescence-guided surgery.
18:00 - 19:00: Challenges and Future of Fluorescence-Guided Surgery (GUSTAVO JACOB)
• Discussion on the obstacles in using fluorophores, interpreting fluorescence signals, and the clinical introduction of new technologies.

Day 2: Advances, Live Practice, and Clinical Integration

8:00 - 12:00: Live Surgeries with Interaction
• Live surgeries focusing on different specialties (cholecystectomy, breast, melanoma, hysterectomy, endometriosis, colorectal anastomosis), allowing real-time interaction and discussion.
10:00 - 10:30: Coffee Break During Surgeries
12:00 - 12:30: Discussion and Experience Sharing
12:30 - 14:00: Lunch
14:00 - 15:30: Advanced Applications and Innovations
14:00 - 14:30: Overview of commercially available fluorophores and camera systems, ongoing studies. (BRUNO AZEVEDO)
15:00 - 15:30: Future of fluorescence signal quantification and clinical translation. (GUSTAVO JACOB)
15:30 - 16:00: Discussion and Questions
16:00 - 16:30: Coffee Break
16:30 - 17:30: Interactive Sessions and Group Discussions (BRUNO AZEVEDO and GUSTAVO JACOB)
• Case analysis, group discussions, and experience sharing on integrating fluorescence-guided surgery into clinical practice.
17:30 - 18:00: Conclusion and Future of Fluorescence-Guided Surgery (BRUNO AZEVEDO)
• Discussion on the future of the technology, potential research areas, and development.
18:00 - 18:30: Closing Session, Certificate Distribution and Feedback