Indocyanine green (ICG) fluorescence imaging is widely used in cervical cancer surgery for sentinel lymph node mapping and lymphatic visualization. This technique enables accurate identification of nodal drainage pathways, reducing the need for extensive lymphadenectomy and associated morbidity.
In the EMEA region, standardized dosing and timing protocols help ensure consistent fluorescence imaging, improving detection rates and supporting intraoperative decision-making in gynecologic oncology.
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Region of interest
, Contenido:
Sentinel Lymph Node (SLN)
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Dose
, Contenido:
0.25 mL (2.5 mg/mL)
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Route
, Contenido:
Submucosal peritumorally at 2,4, 8 and 10 o’clock
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Injection time
, Contenido:
Onset of surgery
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First ICG detection
, Contenido:
After 10 min
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ICG Duration
, Contenido:
Remains visible during procedure as it diffuses slowly via lymphatics
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Camera requirements
, Contenido:
Laparoscope, NIR light source camera
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References (full ref details at end of this guide doc)
Indocyanine green (ICG) fluorescence imaging is widely used in endometrial cancer surgery for sentinel lymph node mapping and lymphatic visualization. This technique enables accurate identification of nodal drainage pathways, supporting precise staging while reducing the need for extensive lymphadenectomy and associated morbidity.
ICG-based near-infrared fluorescence has demonstrated high detection rates and strong diagnostic performance, improving bilateral sentinel lymph node identification and intraoperative decision-making.
In the EMEA region, standardized dosing and timing protocols help ensure consistent fluorescence imaging and optimize surgical outcomes in gynecologic oncology.
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Region of interest
, Contenido:
SLN mapping
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Dose
, Contenido:
2 mL (1.25 mg/mL)
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Route
, Contenido:
Combined cervical (1 mL) and submucosal (1 mL) deep (1-2 cm) into the cervical submucosa
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Injection time
, Contenido:
By time trocars get placed
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First ICG detection
, Contenido:
After 10 mins
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ICG Duration
, Contenido:
Remains visible during procedure as it diffuses slowly through lymphatics
Columna1:
Camera requirements
, Contenido:
Laparoscope, NIR light source camera
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References (full ref details at end of this guide doc)
Indocyanine green (ICG) fluorescence imaging is increasingly used in complex procedures such as hysterectomy, colorectal, pelvic, and abdominal vascular surgery to enhance visualization of anatomy and assess tissue perfusion. This technique enables real-time identification of critical structures—including ureters, vessels, and lymphatics—supporting safer dissection and reducing the risk of intraoperative complications.
ICG fluorescence also supports evaluation of bowel and organ perfusion, helping surgeons optimize resection strategies and improve surgical precision in highly demanding procedures.
In the EMEA region, standardized dosing and timing protocols help ensure consistent fluorescence imaging and enhance intraoperative decision-making across multidisciplinary surgical settings.
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Region of interest
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Ureter localisation
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Dose
, Contenido:
2.5 mg/mL
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Route
, Contenido:
5 mL into each ureteric catheter
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Injection time
, Contenido:
Prior to procedure
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First ICG detection
, Contenido:
Beginning of surgery
Columna1:
ICG Duration
, Contenido:
Remains visible during procedure
Columna1:
Camera requirements
, Contenido:
Laparoscope, NIR light source camera
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References (full ref details at end of this guide doc)
Indocyanine green (ICG) fluorescence imaging is increasingly used in vulvar cancer surgery for sentinel lymph node mapping and lymphatic visualization. This technique enables accurate identification of inguinal lymphatic drainage pathways, which are critical for staging and prognosis.
ICG-based near-infrared imaging has demonstrated detection rates comparable to conventional techniques such as technetium-99m and blue dye, while reducing procedural complexity and morbidity associated with extensive lymphadenectomy.
In the EMEA region, standardized dosing and timing protocols help ensure consistent fluorescence imaging and support precise, minimally invasive surgical approaches in vulvar cancer management.
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Tabla:
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Region of interest
, Contenido:
Lymphatic drainage and SLN
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Dose
, Contenido:
2 mL (0.125 mg/mL)
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Route
, Contenido:
Peritumoral injection - four quadrants
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Injection time
, Contenido:
3-20 hr before procedure
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First ICG detection
, Contenido:
After 10mins
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ICG Duration
, Contenido:
Remains visible during procedure as it diffuses slowly via lymphatics
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Camera requirements
, Contenido:
Open procedure
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References (full ref details at end of this guide doc)
Indocyanine green (ICG) fluorescence imaging is widely used in gynecologic oncology to visualize lymphatic drainage and identify sentinel lymph nodes during cervical and endometrial cancer surgery. Proper dosing, injection site, and timing of ICG administration are critical to achieve reliable near-infrared fluorescence imaging and accurate lymphatic mapping.
This dosing guide outlines recommended ICG administration techniques for cervical and endometrial cancer procedures, including injection into the cervical submucosa and deep stromal tissue, dosage per quadrant, and expected fluorescence detection during sentinel lymph node mapping.
Administration of the dye should be performed at the level of the cervical submucosa, deep into the stroma. The dye should be distributed into 4 cervical quadrants.
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Dilution (25 mg in 10 mL of sterile water - 2.5mg/mL):
, Contenido:
Yes
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Requires Flush with Sterile Water:
, Contenido:
No
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Proposed Dosage:
, Contenido:
1 mL
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Injection Time:
, Contenido:
Prior to dissection & insertion of uterine manipulator
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First Indocyanine Green Detection:
, Contenido:
At start of procedure
Columna1:
Indocyanine Green Duration:
, Contenido:
Stable during surgery. Slowly diffuses through lymphatics
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Camera Requirements (handheld device, laparoscope or both):
Indocyanine green (ICG) fluorescence imaging is increasingly used during abdominal and pelvic surgery to assist in ureter localization and help prevent inadvertent ureteral injury. With near-infrared fluorescence imaging, surgeons can visualize ureteral pathways in real time, improving anatomical identification during complex surgical procedures.
This dosing and timing chart provides guidance on recommended ICG administration techniques for ureter localization, including injection methods, dosage considerations, and expected fluorescence detection timing to support safe and effective fluorescence-guided surgery.
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Title: Dosing & Timing Chart, Tabla:
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Purpose:
, Contenido:
Visualization of ureters
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Injection Type:
, Contenido:
Other
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Description:
, Contenido:
Cystoscopy guidance is recommended for retrograde intraureteral dye administration
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Dilution (25 mg in 10 mL of sterile water - 2.5mg/mL):
, Contenido:
Yes
Columna1:
Requires Flush with Sterile Water:
, Contenido:
No
Columna1:
Proposed Dosage:
, Contenido:
5 mL per ureter
Columna1:
Injection Time:
, Contenido:
Prior to pelvic dissection
Columna1:
First Indocyanine Green Detection:
, Contenido:
During pelvic dissecction
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Indocyanine Green Duration:
, Contenido:
Remains stable during surgery
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Camera Requirements (handheld device, laparoscope or both):
, Contenido:
Both
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Tips & Tricks:
, Contenido:
Advance ureteral catheter to ensure comprehensive assessment of the ureteral course
Indocyanine green (ICG) fluorescence imaging is increasingly used in vulvar cancer surgery to visualize lymphatic drainage and identify sentinel lymph nodes. With near-infrared fluorescence imaging, surgeons can track lymphatic pathways in real time, improving sentinel lymph node detection and supporting more precise oncologic staging.
This dosing chart provides guidance on recommended ICG administration for vulvar cancer procedures, including peritumoral injection techniques, dosage per injection site, and expected fluorescence detection timing to optimize sentinel lymph node mapping during fluorescence-guided surgery.
Título del video: Ureter Identification in Complex Endometriosis
Chapter: EUROPEAN CHAPTER
Speakers: Manish Chand MD, PhD
Descripción: Dr. Manish Chand, discusses using retrograde ICG for ureter identification in complex endometriosis, with a multidisciplinary team approach of colorectal, urologic, and gynecologic surgeons.